摘要
目的:本实验通过观察卵巢切除和衰老对基底前脑的内侧隔核和斜角带水平肢的乙酰胆碱转移酶胆碱能神经元变化,来探讨卵巢切除和衰老对基底前脑的胆碱能神经元胞体和突起的影响。方法:通过利用乙酰胆碱转移酶免疫组织化学的方法观察正常成年雌性组、切除卵巢组和老年组的动物胆碱能神经元形态和突起的变化。结果:切除卵巢组的动物的胆碱能神经元的细胞数(237±8),细胞大小(100.86±37.01)μm2,突起长度(100.86±37.01)与正常成年雌性组之间有明显的差异性(t=4.82～33.08,P<0.05)。正常雌性组和老年组的乙酰胆碱转移酶的细胞数(320±11,185±12;220±14,164±11),细胞大小(172±63,200±69;125±38,129±44),突出数目(1.17±0.45,1.64±0.23;0.76±0.45,1.17±0.42)有明显的差异性(t=9.11～31.02,P<0.05)。结论:切除卵巢和衰老能够引起基底前脑的胆碱能神经元胞体大小、数目及其突起数目的明显减少,这有可能解释衰老或神经退行性疾病导致的认知功能障碍。
AIM:To observe the degeneration of choline acetyltransferase(ChAT)neurons in the basal forebrain due to ovariectomy and aging so as to explore the effects of ovariectomy or aging o n ChAT neurons and their cell processes.METHODS:Immunocytochemical staining with s pecific antibody against ChAT was used to examine ChAT-immunoreativity of neurons and their cell processes in the basal forebrain of y oung and aged female rats and ovariec-tomized rats.RESULTS:A significant reduction occurred in t he number(237±8),size(100.86±37.01)μm 2 ]
出处
《中国临床康复》
CSCD
2003年第28期3826-3827,T002,共3页
Chinese Journal of Clinical Rehabilitation
关键词
卵巢切除
衰老
基底前脑
胆碱能神经元
认知功能
SignificantmorphologicalchangesofChATimmunoreac-tiveneuronsinthebasalforebrainandtheircellprocessesoccurino-variectomizedandagedrats.Thesefindingsmayprovideevidencetoexplaincognitivedeficitinducedbyagingandneurodegenerativediseases.
