人膀胱癌组织中细胞外基质金属蛋白酶诱导因子的表达

Expression of Extracellular Matrix Metalloproteinase Inducer in Human Bladder Transitional Cell Carcinoma

背景与目的:基质金属蛋白酶(matrix metalloproteinases,MMPs)在恶性肿瘤的发生、浸润和转移中发挥关键作用,肿瘤细胞表达的细胞外基质金属蛋白酶诱导因子(extracellular matrix metalloproteinase inducer,EMMPRIN)司以诱导间质成纤维细胞合成MMPs,从而促进肿瘤的浸润和转移。本研究拟通过检测EMMPRIN在人膀胱癌组织和正常膀胱粘膜中的表达,探讨EMMPRIN在膀胱癌发生、浸润和转移中的作用。方法:应用免疫组化SP方法检测EMMPRIN在45例膀胱癌组织和9例正常膀胱粘膜中的表达,并与临床病理指标结合起来分析。结果;EMMPRIN蛋白定位于癌细胞的胞膜和胞浆,阳性率为73.3％,正常膀胱粘膜和肿瘤间质均为阴性表达。EMMPRIN在Ⅱ、Ⅲ级和术后复发组的阳性表达率分别为84.0％、84.6％和100％,高于Ⅰ级和无复发组(分别为14.3％和55.6％)(P<0.05);在淋巴结转移组的强阳性表达率(85.7％)高于无淋巴结转移组(23.7％)(P<0.05)。结论:EMMPRIN在人膀胱癌组织中过度表达,与膀胱癌的恶性程度有关,可作为判断膀胱 癌是否具备高侵袭转移潜能的指标。

BACKGROUND & OBJECTIVE: Matrix metalloproteinases(MMPs) play critical roles in the process of tumorigenesis, tumor invasion and metastasis. Extracellular matrix metalloproteinase inducer (EMMPRIN) derived from cancer cell can induce stromal fibroblasts to synthesize MMPs, which advances the invasion and metastasis of cancer. This study was designed to investigate the roles of EMMPRIN in the tumorigenesis, invasion and metastasis of bladder carcinoma by detecting the expression of EMMPRIN in human normal and carcinomatous bladder tissues. METHODS: The expression of EMMPRIN was examined in 45 bladder carcinoma tissues from 45 patients and normal bladder mucosa from 9 persons by immunohistochemistry SP method. The relationship between EMMPRIN expression and clinicopathologic parameters was analyzed. RESULTS: EMMPRIN protein was localized at membrane and cytoplasm of carcinomatous epithelial cell, but was not expressed in normal bladder epithelial or tumor stromal cell. The overall positive rate of EMMPRIN in 45 cases of bladder carcinoma was 73%. The positive rates of EMMPRIN in grade II, grade III and recurrent carcinomas were 84% , 85% , and 100% , respectively, significantly higher than those in grade I and non-recurrent carcinomas (14% and 56% , respectively) ( P <0. 05). The intensive positive rate of EMMPRIN in lymph node metastasis group (86% ) was significantly higher than that in no lymph node metastasis group (24%) (P<0. 05). CONCLUSION: EMMPRIN was overexpressed in human bladder transitional cell carcinoma, and related to the malignant degree and recurrence. It could be a useful indicator for the assessment of potent invasion and metastasis of bladder carcinoma.