aFGF和bFGF在卵巢癌中的表达及对细胞增殖的影响

Expression of aFGF and bFGF in Ovarian Cancer and Their Effect on Ovarian Cancer Cell Proliferation

背景与目的:近年研究发现,成纤维细胞生长因子(fibroblast growthfactor,FGF)在多种恶性肿瘤中均有过度表达,在卵巢癌中已发现存在高表达的碱性FGF(basic FGF,bFGF),但在原发性卵巢癌中,尚未见有关酸性FGF(acidic FGF,aFGF)的报道。本研究拟检测卵巢癌组织中aFGF和bFGF的表达,以及外源性aFGF和bFGF对卵巢癌细胞株OVCA3细胞增殖的影响,以探讨aFGF和bFGF在卵巢癌发生、发展中的意义。方法:应用逆转录-聚合酶链反应技术(reverse transcriptionpolymerase chain reaction,RT-PCR)对40例上皮性卵巢癌组织中aFGF mRNA和bFGF mRNA的表达进行分析;通过MTT比色法,观察aFGF和bFGF对卵巢癌细胞增殖的影响。结果:aFGF mRNA和bFGF mRNA在上皮性卵巢癌组织中分别为0.981±0.130和1.023±0.131,与正常卵巢(0.636±0.084和0.724±0.079)、卵巢瘤样病变(0.696±0.067和0.742±0.094)及卵巢良性肿瘤(0.694±0.106和 0.737±0.089)相比,差异有统计学意义(P<0.05),在Ⅲ～Ⅳ期卵巢癌中的表达水平明显高于Ⅰ～Ⅱ期。OVCA3细胞株中加入外源性aFGF和bFGF后,增殖比明显增加,并与aFGF和bFGF浓度呈显著正相关。当aFGF浓度为1.2μg/ml时,OVCA3细胞增殖比为对照组的1.51倍;bFGF浓度为100ng/ml时,OVCA3细胞增殖比为对照组的1.85倍。结?

BACKGROUND & OBJECTIVE: There was overexpression of fibroblast growth factor (FGF) in many kinds of malignant tumors. Overexpression of basic fibroblast growth factor (bFGF) has been found in ovary cancer and there is no report about acidic fibroblast growth factor (aFGF) in ovary cancer up to now. The aim of this study was to explore the expression of aFGF and bFGF in ovarian cancer, and the effects of exogenous aFGF and bFGF on the proliferation of ovarian cancer OVCA3 cells, to discuss the relationship between FGF and ovary cancer. METHODS: The expression levels of aFGF and bFGF were determined by reverse transcription polymerase chain reaction (RT-PCR) in 40 cases of ovarian epithelial cancer. MTT assay was used to determine the influence of aFGF and bFGF on the proliferation of ovarian cancer cell. RESULTS: The expression levels of aFGF mRNA and bFGF mRNA in the ovarian epithelial cancer were 0. 981±0. 130 and 1. 023±0. 131, respectively. Compared with normal ovary, ovarian tumor like lesion and benign ovary tumors, the difference was significant ( P <0. 05). The expression levels in stage Ⅲ - Ⅳ were significantly higher than those in stageⅠ -Ⅱ. Addition of exogenous aFGF and bFGF to OVCA3 cell line resulted in significant and reproducible increases in cell number in a dose-dependent manner. When the concentration of aFGF was at 1. 2 μg/ml, the proliferative ratio of OVCA3 cell line was 1. 51 times of the control group. When the concentration of bFGF was at 100 ng/ml, the proliferative ratio of OVCA3 cell line was 1.85 times of the control group. CONCLUSION: aFGF and bFGF may play important roles in carcinogenesis, development, and invasion of ovarian epithelial cancer, and they can be used as the important indices for biologic behavior of ovarian cancer.