IL-2基因修饰的肿瘤细胞对小鼠体内巨噬细胞数量和功能的影响

Effects of IL-2 Gene Modified Tumor Cells on the Number and Functions of Peritoneal Macrophages in Vivo

目的 探讨IL - 2基因修饰的肿瘤细胞对小鼠体内巨噬细胞 (M)数量和功能的影响 .方法 应用腺病毒载体介导的小鼠IL - 2基因 (Ad -mIL - 2 )修饰CT2 6小鼠结肠腺癌细胞 (CT2 6 -mIL - 2 )后皮下接种小鼠 ,计数小鼠腹腔M的数量 ,观察其吞噬功能 ,混合淋巴细胞反应法 (MLR)测定其抗原提呈能力 ,MTT法检测其杀伤活性 .结果 mIL - 2基因修饰的CT2 6细胞 (CT2 6 -mIL - 2细胞 )皮下接种后 ,小鼠腹腔M数量显著增加 ,吞噬能力明显增强 ,抗原提呈能力提高 ,并具有较强的杀伤活性 .结论 CT2 6 -mIL - 2细胞分泌的IL - 2能有效地激活M ,这可能是CT2 6 -mIL - 2细胞体内致瘤性下降的原因之一 .

Objective To observe the effects of IL-2 gene modified tumor cells on the number and functions of murine peritoneal macrophages in vivo. Methods CT26 murine colorectal adenocarcinoma cells were transfected with mIL-2 recombinant adenovirus and tumors were established by S.C. implantation of wild CT26 cells or CT26-LacZ cells or CT26-mIL-2 cells. The number of tumor-bearing mice peritoneal macrophage was calculated and the phagocytic activity was observed. The antigen presenting activity of M was detected by mixed lymphocyte reaction (MLR) assay and the cytoxicity of M was measured by MTT assay. Results After inoculation of CT26-mIL-2 cells, the number of peritoneal macrophages increased significantly. The phagocytic activity, the antigen presenting activity and the cytoxicity of M were all augmental strikingly as compared with the control group. Conclusions IL-2 secreted by CT26-mIL-2 cells in vivo has effectively activated the peritoneal macrophages to some extent, which may account for the decrease tumorigenicity of the