葫芦茶的质量研究

Study on the Quality of Herba Tadehagi Triquetri

目的 建立葫芦茶的质量控制方法。 方法 用薄层色谱法对葫芦茶中的芦丁进行定性鉴别 ;用紫外分光光度法测定葫芦茶中总黄酮的含量。结果 薄层色谱中斑点清晰 ,易于识别 ;紫外分光光度法精密度、重现性良好 ;芦丁在 9.2μg· m L- 1 ～ 5 5 .2μg· m L- 1 范围内呈线性关系 ,r= 0 .9998。平均加样回收率 98.86%,RSD=2 .2 1%( n=9)。 结论

OBJECTIVE To establish a method for quality control of Herba tadehagi triquetri.METHODS Rutin in Herba tadehagi triquetri was identified by TLC;Determination of total flavone content of Herba tadehagi triquetri by UV was reported.RESULTS The spots in the TLC were clear and to be distinguished well.The precision and reproducibility of UV were good.The linear of rutin was within9 2μg·mL -1 ～55 2μg·mL -1 ,r=0 9998,the average recov-ery was 98 86%,and the RSD was 2 21%(n=9).CONCLUSION This method can be used for the qulity control of Herba tadehagi triquetri.