子宫内膜异位症大鼠模型的免疫功能研究

Immunology study on rat model of endometriosis

目的 采用子宫内膜异位症 (内异症 )大鼠模型 ,探讨模型鼠的自然杀伤细胞 (NK)活性及数目的变化 ,及这一变化能否通过输注方式传给正常大鼠。方法 将雌鼠子宫移植于肠系膜制成子宫内膜异位症模型 ,4周后切除模型鼠脾脏和剩余子宫。将去除单核细胞后的脾细胞悬液通过尾静脉注入正常鼠 ,4d后测定其NK细胞活性 ,同时用免疫组化法检测子宫中的免疫细胞。共分 4组 :未作任何处理的对照组 (A组 ) ,脾细胞注射对照组 (B组 ) ,异位症模型组(C组 ) ,接受脾细胞注射组 (D组 )。结果 与A组相比 ,C组脾NK细胞活性降至 42 %,而D组降至 3 9%(P <0 0 0 1)。免疫组化方面 ,C组和D组NK细胞数与A组相比分别下降至 62 %和 5 5 %(P <0 0 0 1)。结论 内异症模型鼠脾脏NK细胞活性下降 ,内膜中NK细胞数减少。

Objective To study the changes in the number and activity of natural killer(NK) cell using an endometriosis rat model and whether the abnormal immune reaction in the model rat can be transferred to normal rat. Methods An endometriosis model was prepared using 8 weeks old female rats by grafting a small section of one uterine horn onto the mesentery, followed by removal of the spleen and the remaining uterine horn 4 weeks later. Splenocytes of the model rats were injected to the normal rats via the tail vein. NK cell activity was assayed 4 days later. The uterus was investigated immunohistochemically for immune cells. There were 4 groups: untreated control group(group A), control-splenocyte injection group(group B), endometriosis model group(group C) and endometriosis model splenocyte injection group(group D). Results NK cell activity decreased in group C to 42% of group A and to 39% in group D. The number of NK cells in group C and D decreased to 62% and 55% of group A, respectively. Conclusion The NK cell number and activity of model rat decreased. The same abnormal immunity can recur in a normal rat after receiving splenocyte transfusion.