胃肠道腺癌患者血清抗癌胚抗原抗体的检测

The presence of anti-carcinoembryonic antigen (CEA) antibodies in the sera of patients with gastrointestinal malignancies and it's diagnostic significance

目的 探讨胃肠道腺癌患者血清中IgG和IgM抗癌胚抗原 (CEA)抗体的情况 ,评价CEA及IgG和IgM抗CEA抗体的联合检测在胃肠道腺癌诊断中的意义。方法 用放免法检测血清中CEA含量 ,酶联免疫吸附法 (ELISA)检测血清中IgG和IgM抗CEA抗体 ,用竞争抑制法检测抗体的特异性。结果 胃肠道腺癌患者血清CEA含量升高者 (≥15ng/ml)占 3 0 9%( 2 1/68) ,IgG抗CEA抗体阳性者占 3 5 3 %( 2 4/68) ,IgM抗CEA抗体阳性者占 2 6 5 %( 18/68) ,CEA及抗CEA抗体的联合检测可使胃肠道腺癌的阳性率提高到 60 3 %( 4 1/68) ;健康对照组血清CEA升高者为 0 ,IgG和IgM抗CEA抗体阳性者占 3 3 %( 1/3 0 )。结论 胃肠道腺癌患者血清中IgG和IgM抗CEA抗体的检出率较高 。

Objective To determine the Clinical significance of IgG and IgM anti-CEA antibodies in the serum of patients with gastrointestinal malignancies. Methods CEA levels were measured by RIA. The IgG and IgM anti-CEA antibodies were measured by an enzyme-linked immunoassay (ELISA). The specificity of circulating antibodies against CEA was carried out by an inhibition assay.Results CEA was elevated (≥15 ng/ml) in 21 patients with gastrointestinal malignancies (30 9%) and not in healthy donors. IgG anti-CEA antibodies were detected in 24 patients (35 3%) and IgM anti-CEA antibodies were detected in 18 patients (26 5%) with gastrointestinal malignancies. These antibodies can also be detected in 1 health individual (3 3%). Conclusion High percentage of gastrointestinal malignancies patients have circulating CEA specific antibodies in their sera and these antibodies may be used as diagnostic markers.