HPLC法测定活血通脉片中丹参酮ⅡA的含量

Quantitative Determination of Tanshinone ⅡA in Huoxuetongmai Tablet by HPLC

目的 :测定活血通脉片中丹参酮ⅡA的含量。方法 :HPLC法 :以C1 8为填充柱 ,甲醇 水 (80∶2 0 )为流动相。检测波长2 6 8nm。结果 :丹参酮ⅡA在 0 2 μg～ 1 2 μg范围内线性关系良好 ,平均回收率为 98 5 3% ,RSD =1 0 8%。结论 :本法简便、快速、准确 ,可作为活血通脉片的质量控制方法。

Objective\ To develop a method for the determination of Tanshinone ⅡA in Huoxuetongmai tablet.Methods\ HPLC:Kromasil C 18 and methanol-water(80∶20)were used as the chromatographic column and the mobile phase respectively with the detected wavelength at 268nm.Results\ The linearity was obtained over the range of 0\^2μg～1\^2μg.The average recovery was 98\^53%( RSD =1\^08%).Conclusion\ The established method is simple,rapid,accurate and suitable for the quality control of Huoxuetongmai tablet.