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长白山赤杨丛枝菌根真菌多样性的半巢式LP-PCR-SSCP检测分析 被引量:5

Semi-nested LP-PCR-SSCP identification of arbuscular endomycorrhizal fungi diversity of Alnus at Changbai Mountain
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摘要 利用半巢式LP PCR SSCP技术 ,对中国吉林长白山不同海拔生境下 3种赤杨共生丛枝菌根真菌的多样性进行检测分析 .结果表明 ,该地区赤杨属东北赤杨、西伯利亚赤杨及色赤杨共生丛枝菌根真菌在科乃至种的水平上并未随宿主的变化表现出丰富的多样性 ;3个树种在自身属的水平上与共生的球囊霉科 (Glomaceae)至少 1种AMF ,即G .intraradix ,在种的水平上表现出不相关于宿主海拔高度的某种相互选择性 . The diversity of arbuscular endomycorrhizal fungi colonized on three species of Alnus at Changbai Mountain was identified based on coupling the sensitivity of semi-nested LP-PCR and the specificity afforded by Single Strand Conformation Polymorphism analysis. It is suggested that altitudes and species of Alnus were not the important factors deciding the distribution of AMF in the grade of family, and there was at least one species of Glomaceae, Glomus intraradix, dominated in Alnus at Changbai Mountain.
出处 《应用生态学报》 CAS CSCD 2003年第11期1837-1841,共5页 Chinese Journal of Applied Ecology
基金 国家重点基础研究发展规划项目(0 0 1CB10 89) 中国科学院长白山森林生态系统定位研究站基金资助项目
关键词 赤杨 丛枝菌根真菌 半巢式LP-PCR-SSCP Alnus, Arbuscular endomycorrhizal fungi, Semi-nested LP-PCR-SSCP
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