凯西莱抗小鼠过氧化肝损伤的作用

Antioxidant and hepatoprotective effects of tiopronin in mice

目的 研究凯西莱抗小鼠过氧化肝损伤的作用。方法 采用四氯化碳 (CCl4)腹腔注射制备小鼠急性肝损伤模型 ,以凯西莱 50mg/kg治疗 ,每日 1次。连续治疗 4天后 ,检测血清丙氨酸氨基转移酶 (ALT)和丙二醛 (MDA)水平及肝脏MDA、还原型谷胱甘肽 (GSH)和超氧化物歧化酶 (SOD)水平 ,观察肝组织病理变化。结果 与正常组相比较 ,CCl4 模型组血清ALT、MDA和肝脏MDA水平均显著增高 (P <0 .0 1) ,肝脏GSH和SOD水平则显著降低 (P <0 .0 1) ,同时肝组织病理损伤明显。凯西莱可显著降低血清ALT和MDA水平及肝组织MDA含量 (P <0 .0 1) ,使肝脏GSH和SOD恢复至正常水平 (P >0 .0 5) ,且能明显减轻肝组织病理损伤。相关分析显示 ,血清MDA与肝组织MDA、血清ALT水平间均呈高度直线相关。结论 凯西莱具有良好的抗CCl4 所致的小鼠过氧化肝损伤作用 ;

ObjectiveTo study the antioxidant and h epatoprotective effects of tiopronin in mice.MethodsAcute hepatic damage in mice was induced by injection of carbon tetrachloride(CCl 4) intraperitoneally,and the mice were treated with tiopronin at the dose of 50 mg·kg -1 ·d -1 for 4 days.Serum alanine a minotransferase (ALT) and malondialdehyde(MDA) levels were measured,and hepatic MDA,reduced glutathione(GSH) and superoxide dismutase(SOD) levels were also dete rmined.Histopathological changes of liver were observed with microscope.ResultsCompared with normal control mice,CCl 4 treated-mice man ifested significant increase of serum ALT,MDA and hepatic MDA( P<0.01), while hepatic GSH and SOD were significantly decreased in the CCl 4 treated -mice( P<0.01). Significantly histopathological damage of the liver w a s found in the CCl 4 treated-group.In the tiopronin therapeutic groups,serum ALT,MDA and hepatic MDA were significantly lower than those in the CCL 4 model groups( P<0.01), and hepatic GSH and SOD were returned to normal( P >0.05), and hepatic damage was also significantly improved. There were closely linear correlations between serum MDA and hepatic MDA,and bet ween serum MDA and serum ALT( P<0.01) .ConclusionTiopronin possesses potent antioxidant and hepatoprote c tive effects in mice induced by carbon tertralhloride,and serum MDA can reflec t the hepatic damage caused by peroxidation.