脑脉康对动脉粥样硬化家兔血小板聚集率和病理形态学的影响

Effects of Naomaikang on the Platelet Aggregation Rate and Pathomorphological Changes of Experimental Atherosclerosis Rabbits

目的 :探讨脑脉康抗动脉粥样硬化 (AS)的作用。方法 :将家兔随机分为空白组、模型组、非诺贝特组、脑脉康大剂量组、脑脉康小剂量组 ,每组10只。模型组饲喂胆固醇建立AS模型 ,其它组药物干预。实验前、后测定血小板聚集率 (PAR) ,主动脉弓分别固定送光镜和电镜检查。主动脉油红O染色 ,计算斑块平均面积和斑块率。结果 :实验后模型组PAR显著增高 ,与各组比较均有显著性差异 (P<0 01) ;病理形态学观察显示 ,模型组斑块率为66 51 % ,非诺贝特组为49 39 % ,脑脉康大、小剂量组分别为37 88 %和40 46 %。光镜和电镜检查显示 ,模型组呈典型AS改变 ,非诺贝特组和脑脉康组AS损害明显减轻。结论

OBJECTIVE:To investigate the inhibiting effect of Naomaikang(NMK) on atherosclerosis(AS) METHODS:Healthy male california rabbits were randomized into 5 groups(n=10):normal group,model group,Fenofibrate group,NMK groupⅠ(2ml) and NMK group Ⅱ(1ml) The rabbits in each group,except the normal one,were fed with hypercholestrol diet and the medicines were given to the other three groups at the same time The blood of each group was sampled for determining the platelet aggregation rate(PAR) before and after experiment The aorta of rabbit in each group was dissected out and stained with oil red 0,then the areas and incidences of plaque on aorta wall were calculated,the aortic arch was taken to observe under the light and electron microscopes RESULTS:The PAR in model group was significantly increased compared with those in other groups(P<0 01) The pathomorphological observation showed that the plaque incidences were 66 51% in the model group,49 39% in the Fenofibrate group,37 88% in the NMK group Ⅰ(2ml) and 40 46% in the NMK group Ⅱ(1ml) group There were typical atherogenic lesion in the model group and less in other groups under the light and electron microscopes CONCLUSION:NMK can markedly inhibit the occurrence and development of AS in many links