661份HIV抗体阳性血浆样本HIV-1 RNA浓度测定

Quantitative Detection of HIV-1 RNA for 661 Anti-HIV-1 Sero-positive Plasma

目的 评价HIV感染者和艾滋病人体内病毒载量水平。方法 用新一代NASBA试剂及其检测系统(NUCLISENSTM HIV 1QT)测定 6 6 1份来自HIV感染者和艾滋病人的抗体阳性血浆样本HIV 1RNA浓度。结果 6 6 1份样本中 ,血浆HIV 1RNA浓度能被NASBA方法定量的样本数为 4 6 9份 (70 .95 % ) ,不能定量 (低于试剂盒可检限 )的样本数为 192份 (2 9.0 5 % )。 4 6 9份能被定量的样本中 ,HIV 1RNA浓度 (cp/ml)≤ 5 0 0、5 0 1～ 30 0 0、30 0 1～ 10 0 0 0、10 0 0 1～ 30 0 0 0、>30 0 0 0的样本百分率分别为 2 .98%、2 0 .90 %、17.70 %、14 .2 9%和 4 4 .13%。结论 血浆HIV 1RNA浓度能被定量的HIV 1感染者和艾滋病人体内病毒载量具有普遍偏高趋势。

Objective To assess the plasma HIV-1 RNA level of HIV-infected persons and AIDS patients.Methods The HIV-1 RNA concentrations of 661 anti-HIV-1 sero-positive specimens from HIV-infected persons and AIDS patients were tested by NUCLISENSTM HIV-1 QT.Results Among 661 specimens,the HIV-1 RNA concentrations in 469 plasma (70.95%) were determined,and with the concentration distributions of 2.98% (≤500 cp/ml),20.90%[(501-3000) cp/ml],17.07%[(3001-10000)cp/ml],14.29%[(10001-30000)cp/ml],44.13% (>30000 cp/ml),respectively.Whereas the HIV-1 RNA concentrations in 192 plasma (29.05%) were under the detection limit value of the method in the test.Conclusion Most of 469 HIV-infected persons and AIDS patients have a high HIV-1 RNA level.