脂欣康胶囊抗载脂蛋白E基因敲除小鼠动脉粥样硬化形成作用

Effect of Zhixinkang Capsule on Atherosclerosis(AS) Formation in Apolipoprotein E-gene Knockout Mice

为观察脂欣康胶囊对载脂蛋白E(ApoE)基因敲除小鼠动脉粥样硬化 (As)的影响 ,将 6周龄ApoE基因敲除小鼠随机分为模型组与脂欣康组 ,相同遗传背景的同龄正常C57BL/6J小鼠为正常对照组。脂欣康组灌服脂欣康胶囊内之药粉 ,模型组、正常对照组均灌服生理盐水。连续灌胃 14周后 ,用 10 %福尔马林灌注后 ,取主动脉做形态学观察及图像分析。结果表明 ,正常对照组主动脉壁正常 ,无As病灶。模型组动脉粥样硬化 (As)病变显著 ,多为粥样硬化期病灶 ,可见由大量泡沫细胞形成的脂纹脂斑期病变 ,也可见由大量泡沫细胞及胆固醇结晶形成的粥样斑块期病灶。脂欣康组As病变较模型组明显减轻 ,多为早期粥样硬化 ,斑块面积显著小于模型组 ,P <0 .0 5。结果提示脂欣康有减轻ApoE基因敲除小鼠As的作用。

To observe the Zhixinkang Capsule's (ZXKC) effect on atherosclerosis (As) of Apolipoprotein E(ApoE)-gene knockout mice,six-week old ApoE-gene knockout mice were assigned randomly into two groups: model group(fed with saline) and ZXKC group (fed with ZXKC). Besides, six-week old healthy mice (C 57 BL/6J mice ) were the normal group (fed with saline). After treating 14 weeks, all the mice were filled with 10% formalin and the aortas were stripped for morphological research and photograph analysis. The photography analysis showed that the lesion of model group was very obvious. Compared with normal group, the total atheromatous plaque square increased obviously (P<0.05). Morphological reseach showed that As lesions were found in aortas of model group and ZXKC group. While As lesions of model group were more serious than those of ZXKC group, and no As lesions were found in normal group.ZXKC can relieve the As lesions of the ApoE-gene knockout mice.