严重烧伤后早期大鼠肝脏细胞粘附分子1和白介素6的表达

Expression of hepatic intercellular adhesion molecule and interleukin 6 in rats after severe burns

目的 :观察严重烧伤早期大鼠肝脏细胞粘附分子 1(Intercellularadhesionmolecule— 1,ICAM - 1)、白细胞介素 6(Interleukin6 ,IL - 6 )在不同时间点的表达及早期病理变化 ,为阐明烧伤早期肝脏损伤机制及为伤后早期损伤的防治打下基础。方法 :(1)光镜和电镜观察大鼠在烧伤后早期 (5min至 72h)肝脏的病理变化。 (2 )免疫组织化学、原位杂交和图橡分析技术对伤后不同时间点肝脏ICAM - 1进行染色和分析。 (3)免疫组织化学、原位杂交和图像分析技术对伤后不同时间点肝脏IL - 6进行染色和分析。结果 :(1)伤后早期主要病理变化为出血、充血和水肿 ;电镜显示 :肝脏毛细血管内皮细胞受损。 (2 )伤后 30min,免疫组化和原位杂交显示ICAM - 1、IL - 6表达增强 ;伤后 2h～2 4h明显增强 ,呈阳性或强阳性 ;伤后 72h ,表达逐渐减弱。结论 :伤后早期肝脏的损伤可能与内皮细胞损伤有关 ,I CAM - 1、IL -

Objective:To observe the expression and early pathologic changes of intercellular adhesion molecule 1(ICAM-1)and interleukin 6(IL-6)in rats after severe burus so as to demonstrate the mechanisms of early hepatic injuries and provide theoretic basis for prevention and treatment.Methods:Light and electron microscopes were employed to observe the early hepatic pathologic changes(5 minutes to 72 hours after burn).The expressions of ICAM-1 and IL-6 were observed by immunocytochemistry,in situ hybridization and image analysis.Results:Main early pathologic changes in liver were edema,hemorrhage and congestion.Injuries of the epithelium cells of capillary vessels were observed by electron microscope.Immunocytochemistry and in situ hybridization showed that ICAM-1 and IL-6 began to increase 30 minutes after burns,significantly increased 2 hours to 24 hours later, and gradually decreased 72 hours later.Conclusion:ICAM-1 and IL-6 may play an important role in early hepatic injuries,and the major target cell may be the endothelium cell.