叶酸缺乏对乳腺癌化疗疗效和毒性的影响

The effect of folacin deficiency on the chemotherapy of breast cancer

目的 :检测叶酸缺乏时化疗药物对人乳腺细胞株HBL 1 0 0和人乳腺癌细胞株MCF 7敏感性的影响。 方法 :将HBL 1 0 0和MCF 7在正常培养液和叶酸缺乏培养液中加入化疗药物 ,培养 3天 ,用四唑蓝 (MTT)法检测化疗药物对HBL 1 0 0和MCF 7的杀伤作用。 结果 :MCF 7对阿霉素 (ADM)、顺铂 (DDP)、氟尿嘧啶 (5 FU)、丝裂霉素(MMC)和甲氨蝶呤 (MTX)等化疗药物均不敏感。将 5 FU、MMC和MTX分别加入实验组和对照组培养液后 ,对照组中MCF 7细胞数减少 ,光密度值降低。ADM和DDP对MCF 7生长的抑制作用在两种培养液中无显著差异。ADM、DDP、5 FU、MMC和MTX等化疗药物 ,在两种培养液中 ,对HBL 1 0 0光密度值的影响无显著差异。 结论 :叶酸缺乏可以降低某些化疗药物如 5 FU、MMC和MTX对MCF 7的杀伤作用 ,但不改变ADM、DDP、5 FU、MMC和MTX等化疗药物对HBL 1 0

Objectives:To determine the effect of folacin deficiency on the chemotherapy of breast cancer lines MCF 7 and breast cell line HBL 100. Methods:These two cell lines were cultured for 3 days in the presence of chemotherapeutics.The MTT method was used for determination. Results: MCF 7 was not sensitive to ADM,DDP,5 FU,MMC or MTX. Treatment in vitro , there was no difference in cell numbers. Added with 5 FU,MMC or MTX, folacin deficiency decreased the growth of MCF 7. Folacin deficiency did not influence the effect of ADM,DDP,5 FU,MMC or MTX on HBL 100. Conclusions:Folacin synergizes the effect of 5 FU, MMC and MTX on the MCF 7 cell line.In the normal therapeutic range of ADM, DDP, 5 FU, MMC and MTX,folacin dificiency does not increase the toxic effects on the HBL 100 cell line.