单核细胞与内皮细胞的相互作用及其促细胞因子分泌的机制

The Effects of Monocyte-Endothelium Interaction on the Secretion of Tumor Necrosis Factor-α, Interleukin-1β and Macrophage Colony-stimulating Factor and Its Mechanism Mediated by Adhesion Molecules

为研究单核细胞、内皮细胞的相互作用对肿瘤坏死因子α、白细胞介素 1β和巨噬细胞集落刺激因子分泌的影响以及粘附分子在其中的作用 ,采用单核细胞、内皮细胞单独培养及混合培养的方法 ,在混合培养条件下预加入细胞间粘附分子 1、血管细胞粘附分子 1和内皮选择素的单克隆抗体 ,通过酶联免疫吸附试验检测培养液中肿瘤坏死因子α、白细胞介素 1β和巨噬细胞集落刺激因子的含量。结果发现 ,单核细胞和内皮细胞单独培养条件下观测不到肿瘤坏死因子α、白细胞介素 1β和巨噬细胞集落刺激因子的分泌 ,两种细胞混合培养时 ,3种细胞因子的分泌显著升高 (P <0 .0 5 ) ,细胞间的接触粘附起着重要介导作用 ,内皮选择素在促使肿瘤坏死因子α、白细胞介素 1β分泌的环节中占据主导地位 ,细胞间粘附分子 1在促使巨噬细胞集落刺激因子分泌的机制中占据主导地位。表明单核细胞与内皮细胞的粘附能够激活某些基因的信号转导途径 ,使肿瘤坏死因子α、白细胞介素 1β和巨噬细胞集落刺激因子的分泌增加 ,粘附分子在其中起着重要的介导作用。

Aim To examine the effects of monocyte-endothelium interaction on the secretion of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and macrophage colony-stimulating factor (M-CSF), and to explore the functions of adhesion molecules in the process. Methods Monocytes and endothelial cells were cultured alone or cocultured to form different cell culture conditions. Pretreatments with monoclonal antibody of human intercelluar adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelium selectin (E-selectin) were carried out in cocultured groups. The levels of TNF-α, IL-1β and M-CSF in culture medium were determined by enzyme linked immune sandwich assay technique. Results There was no secretion of TNF-α, IL-1β and M-CSF observed in monocytes or endothelial cells culture alone. The secretion of three types of cytokines increased significantly when two types of cells were cocultured (P<0.05). Direct contact between monocytes and endothelial cells played an important mediated role in the process. E-selectin played the principal role in activating the secretion of TNF-α and IL-1β, and ICAM-1 was the most principal adhesion molecule to mediate the secretion of M-CSF. Conclusion The adhesion of monocytes to endothelial cells can activate certain signal transduction, resulting in significantly increased secretion of TNF-α,IL-1β and M-CSF. Adhesion molecules play a pivotal role in the process.