D-核糖产生菌转酮醇酶活性分析

The Tansketolase Activity Determination of D-ribose-producing Strain

芽孢杆菌属的转酮醇酶(Transketolase,TKT)变异株可利用培养基中的D-葡萄糖,将其转化成D-核糖分泌于细菌培养基中。阐述了D-核糖产生菌转酮醇酶的活性测定方法,制备了测定所必需的2种酶。

Transketolase mutant of bacillus subtilis can produce D-ribose in medium using D-glucose as carbohydrate. We describe a method of transketolase activity determination, in the same time, we also purify two enzymes which were necessary for transketolase activity determination.