血脂康对血脂和血管内皮细胞活性物质的影响

Effects of Xuezhi Kang on Cholesterol and Activity Substances in Vascular Endothelial Cells

探讨血脂康对高胆固醇饮食家兔血脂和血管内皮细胞活性物质的影响。健康纯种新西兰白兔 30只 ,随机分为对照组 (饲以普通饲料 )、高脂组 (饲以普通饲料 +1.5 %胆固醇 )、治疗组 [饲以普通饲料 +1.5 %胆固醇 +血脂康 0 .8g/ (kg·d) ]。在实验不同阶段 ,观察各组家兔血清脂质、一氧化氮及血浆内皮素、前列环素、血栓素含量及血管内皮细胞病理形态学改变。实验前各组血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、血浆内皮素、血清一氧化氮、血浆血栓素及 6 酮 前列腺素等无明显差异。实验 12周 ,高脂组、血脂康治疗组血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、血浆内皮素、血清一氧化氮、血浆血栓素及 6 酮 前列腺素比值明显高于对照组 ,但治疗组上述指标明显低于高脂组 (P值均 <0 .0 5 ) ;高脂组和治疗组血清一氧化氮低于对照组 (P <0 .0 5 ) ,但治疗组高于高脂组 (P <0 .0 5 )。病理结果显示 :血脂康治疗组主动脉、冠状动脉粥样硬化病变及血管内皮细胞损伤明显轻于高脂组。提示血脂康具有明显的调整脂质代谢和影响血管内皮细胞活性物质生成的作用。

Aim To explore the effects of Xuezhi Kang on cholesterol and functions of endothelial cells in cholesterolfed rabbits. Methods The effects of Xuezhi Kang [0.8 g/(kg·d)] on the functions of endothelial cell during the progression of atherosclerosis were investigated in rabbits fed with 1.5% cholesterol diet in this study. 30 New Zealand white rabbits were divided into three groups (control group, hypercholesterol group and Xuezhi Kang group) during the 12 weeks experiment. Serum lipids, nitrous oxide (NO) and plasma endothelin-1 (ET-1), thromboxane (TX),6-keto-prostaglandin-F 1α (6-keto-PGF 1α) concentrations and histopathological changes of endothelial cells were followed. Results Serum total cholesterol, low density lipoprotein cholesterol, triglyceride, plasma ET-1 concentrations and the ratio of TX/6-keto-PGF 1α in plasma decreased and serum NO level increased in Xuezhi Kang group as compared with those of hypercholesterol group (P<0.05). The area of lipid deposition on the intimal surface of the aorta and coronary arteries was reduced and the ultrastructural injuries of endothelial cells were milder in Xuezhi Kang group. Conclusion Xuezhi Kang had antiatherogenic properties and the protection to the functions of vascular endothelial cells which might be involved in the mechanisms of Xuezhi Kang against atherosclerosis.