雌激素对兔髂动脉球囊内皮剥脱术后内皮增殖的影响

Estradiol Accelerates Functional Endothelial Recoveryin an Experimental Model of Vascular Injury

为探讨雌激素对兔髂动脉球囊损伤后内皮细胞增殖速度的影响 ,将成年雌兔给以卵巢切除及髂动脉球囊损伤内皮 ,分别测定对照组及雌激素组血清雌二醇浓度、内皮增生面积百分比和组织一氧化氮浓度的变化。结果发现 ,与对照组相比 ,雌激素组血清雌二醇浓度明显增高 (P <0 .0 1) ,损伤后内皮细胞增生速度明显加快 (P <0 .0 5 ) ,二者呈高度相关关系 (r=0 .947,P <0 .0 0 1) ,同时血管组织一氧化氮浓度明显增加 (P <0 .0 5 )。以上提示雌激素可促进损伤后内皮细胞增殖 ,加快内皮修复并提高损伤内皮功能 ,从而发挥对内皮细胞的保护作用。

Aim To elucidate the effect of estradiol on the endothelium of rabbits iliac artery after balloon injury. Methods 28 female rabbits were randomly divided into four groups. All rabbits were ovariectomized. The endothelial denudation was performed by utilizing the balloon of percutaneous transluminal coronary angioplasty in rabbit right iliac artety. 2 weeks and 4 weeks after balloon injury of the artery, serum levels of estradiol were measured and reendotheliums were assessed by IBAS 2000 morphometric system. The functions of endothelium were tested by measuring the nitric oxide (NO) levels of injured artery. Results Serum levels of estradiol significantly elevated in estrogen treated groups compared with the control groups(P<0.01). The reendothelialization was more accelerated in estrogen treated groups(P<0.05), the corelationship between them is strikingly. The enhanced NO release were observed in estrogen treated groups(P<0.05). Conclusions Estrogen can accelerate reendothelialization after balloon injury and protect the function of injured vascular endothelial cells, so as to have the effects of antiatherosclerosis.