HDAg与HBsAg/HBcAg在丁型肝炎病人肝组织中的表达

Study on the Expression of HDAg and HBsAg/HBcAg in Liver Tissues of the Patients with Hepatitis D

研究HDAg、HBsAg和HBcAg在丁型肝炎病人肝组织中表达及关系 ,探讨HDV致病机理。应用免疫组化双重染色及连续切片技术 ,检测 79例丁型肝炎病人肝组织HDAg、HBsAg和HBcAg表达 ,以 5 2例乙型肝炎作对照。丁型肝炎HBsAg、HBcAg检出率 (81%、71% )较乙型肝炎 (94%、92 % )低 (P <0 0 5或 0 0 1)。HDAg以肝细胞核表达为主 ,HBsAg以肝细胞浆表达为主 ,HDAg和HBsAg表达强度及阳性细胞分布呈一致性 ,均与肝组织炎症活动和病理损害程度相关 (P <0 0 1)。HBcAg以肝细胞核表达为主 ,HBcAg阳性细胞明显少于HDAg阳性细胞。HDV感染可抑制HBV抗原表达 ;在HDV致病机制中既有HDV的直接细胞毒性作用 ,也有HBV和HDV的协同作用。

WT5”BZ]To explore the relationship between the expression of hepatitis D virus antigen(HDAg) and that of HBsAg/HBcAg in liver tissues of the patients with hepatitis D(HD) HDAg,HBsAg,and HBcAg in Paraffin-embedded liver tissue from 79 liver biopsy or autopsy samples from the patients with HD were studied with immunihistochemical double labelling techniques;meanwhile,compared with the expression of HBsAg/HBcAg in liver tissues of the patients with hepatitis B(HB) The detection rates for HBsAg and HBcAg in liver specimens of HD were 81% and 71%,and were lower than that of hepatitis B(94%,92%) (P<0 05 or 0 01) HDAg was mainly expressed in nuclei of hepatocytes,much of positive cells were obviously aggregated in periportal region especially at the advanceing edges of areas of peicemeal necrosis,moreover,strong expression of HDAg was shown in the bolloming degeneration hepatocytes and hepatocytes near piecemeal necrosis region HBsAg was mainly expressed in cytoplasm of hepatocytes,and positive cells distribution were similar to HDAg positive cells HBcAg was mainly expressed in nuclei of hepatocytes,positive cells distribution were alone or focal type (3-5 cells) Double labelling studies showed those both HDAg and HBsAg might be mostly expressed in the same hepatocytes,and positive cells of HDAg and HBsAg were mostly found to locate in the same area of liver tissus,but HDAg coexisted seldomly with HBcAg,respectively the expression degrees and the positive cells disribution of HDAg and HBsAg were all obviously associated with the grades of pathological damage of liver tissus and necroinflammatory activity(p<0 01) [WT5”BZ]Conclution:[WT5”BZ]HDV infection can inhibit replication of HBV genome or expression of HBV antigen Becides of interactions between HBV and HDV,it is possible that direct cytotoxicity of HDV may play an impotant pathogenetic role in pathogenetic mechanisms of HDV [WT5”HZ]