SP法检测死胎肝组织中HBV表达的初步探讨

General study on the Expression of HBV in the liver tissues of died fetus that puerperal delivered with hepatitis virus B

探讨HBV经母婴传播在胎儿肝组织中表达的高危因素。采集 5 0例乙型肝炎产妇娩下的死胎 ,尸检取肝组织 ,SP法检测HBV ;回访婴母产前静脉血HBV的检测结果。HBcAg颗粒的肝细胞胞浆呈弥漫棕黄色 ,呈点、灶片状分布于肝组织中 ;毛细胆管上皮和管腔内也可见到棕黄色HBcAg颗粒。 2 1/ 5 0例 (4 2 % )死胎肝组织中HBcAg阳性 ,其中静脉血HBV单阳、小三阳和大三阳的婴母分娩的死胎肝组织中HBcAg阳性分别为 3/ 12例(2 5 0 0 % )、2 / 12例 (16 6 7% )、16 / 2 6例 (6 1 5 2 % ) ,前两者比较差异不显著 (P <0 0 5 ) ;第三者与前两者比较差异显著 (P >0 0 0 5 )。婴母静脉血HBV大三阳是乙型肝炎病毒通过母婴传播在胎儿肝组织中表达的高危因素。

WT5”BZ]Study the risk factors that the liver tissue of the died fetus infected by HBV by maternal fetal transmission 50 liver tissues of died fetus that the puerperal delivered with hepatitis virus B were detected HBV by immunocytochemistry(SP) Investigated the detection on HBV in the mother veins before delivery There were HBV in the liver tissues of the died fetus that the puerperal delivered with hepatitis virus B The were mainly in the liver cytoplasm and were not in the nuclei,but they were also in the lumens and epithelium cytoplasm of bile capillaries The positive cells were single or in clusters form distributed over the liver tissues There were 27 cases(54%)with HBcAg positive express in the liver tissues of died fetus that the puerperal delivered with hepatitis virus B In this cases there were 3/12(25 00%)、2/12(16 67%)and 16/26(61 52%)cases with HBcAg positive express in the liver tissues of the died fetus that the puerperal delivered with hepatitis virus B for only one ①(HBsAg,HBeAb,HBcAb)、②(HBsAg+HBeAb+HBcAb)and③(HBsAg+HBeAg+HBcAb)in the mother veins before delivery the comparison between①and②were not statistically significant(P>0 05);But the comparison between ① ②and③were statistically significant(P<0 005) It was the more risk factors for the liver tissue of the died fetus infected by HBV by matemal fetal transmission that HBV(HBsAg+HBeAg+HBcAb)was with positive expression in the mother veins before delivery [WT5”HZ]