广东汉人血管紧张素Ⅱ1型受体基因A-C1166多态性与原发性高血压、冠心病的关系

Correlation between angiotensin Ⅱ type 1 receptor gene A-C 1166 polymorphism and essential hypertension, coronary heart disease in Guangdong hans

目的 :了解血管紧张素 受体 1 (AT1 R)基因 3’端非翻译区 (3′- UTR) A- C1 1 66多态性在广东人群中的分布特点及其与该人群患原发性高血压 (EH)和冠心病 (CAD)的关系。方法 :用 PCR和限制性内切酶酶切技术分析了 1 86例广东汉族大学生、68例 EH患者和 72例 CAD患者 AT1 R基因 A- C1 1 66多态性的基因型和等位基因频率。结果 :该人群A- C 1 1 66基因多态性明显不同于白人 ,与中国北方汉人相似。健康组中没发现 CC型纯合子 ,疾病组中仅 1例 EH患者和1例 CAD的患者为 CC基因型。C 1 1 66等位基因频率 0 .0 4 ,A 1 1 66等位基因频率为 0 .96。疾病组与健康组之间差异无显著性。结论 :AT1 R基因 A- C1 1 66多态性与广东汉族人患 EH和 CAD无关。

Objective:To understand the distribution of 3′ UTR A C 1166 polymorphism of angiotensin Ⅱ type 1 receptor (AT 1R) gene and its correlation to essential hypertension(EH) and coronary heart disease (CAD) in Guangdong Hans.Methods: Sixty eight subjects with EH,72 with CAD and 186 normotensive controls were assayed with PCR technique and restriction endonuclease analysis.Results: There were no significant differences in allele or genotype frequencies among EH,CAD and control subjects.Allelic frequency of C1166 in guangdong Hans was lower than that in Caucasian people (0.04 vs 0.27～0.34).CC homozygotes and AC heterozygotes were uncommon,eg,1 case in EH,1 in CAD and zero in controls,and similar to those in northern Chinese Hans.Conclusion: It is suggested that A C1166 polymorphism of AT 1R gene might not be implicated in EH and CAD in Guangdong Hans.