端粒酶在原发性肝癌组织中的表达

Expression of telomerase in primary hepatocellular carcinoma

目的 :探讨端粒酶在原发性肝细胞癌 (HCC)患者肝组织中的表达及临床意义。方法 :用 TRAP- ELISA法检测45例原发性肝细胞癌和 2 6例癌旁组织以及 7例正常肝组织的端粒酶活性。结果 :45例原发性肝细胞癌组织中有 38例显示端粒酶活性 ,其阳性率为 84.4% ,而 2 6例癌旁组织只有 2例具端粒酶活性 ,阳性率只为 7.6% ;7例正常肝组织端粒酶表达均为阴性 ;结果还显示肝癌组织端粒酶的表达与肿瘤病理分化程度无关。结论 :端粒酶在绝大多数原发性肝细胞癌中表达 ,其活性可能在 HCC的发生和发展中起重要作用 ,有希望成为诊断

Objective:To investigate the expression of telomerase in human primary hepatocellular carcinoma and its clinical significance. Methods: Telomerase activity was examined by TRAP ELISA in 45 patients with primary hepatocellular carcinoma.Results: Telomerase activity was found in 38 of 45(84.4%) malignant liver tissues while 2 of 26(7.6%) adjacent tissues,and not expressed in 7 distal normal samples.There was no significant relation between the telomerase expression and tumor size.differentiation degree,and concomitant HBV infection of hepatocellular carcinoma.Conclusion: Telomerase is expressed in most of the tissues of primary hepatocellular carcinoma,so it probably plays an important role in the tumorigenesis.Telomerase activity would become a prospective diagnostic marker on hepatocellular carcinoma in the near future.