妊高征患者外周血与脐血和羊水血管内皮生长因子的测定及其意义

Assay and Significance of vascular endothelial growth factor in Peripheral Blood,Amniotic Fluid and Cord Blood in Patients with Pregnancy Induced Hypertension

目的 :探讨血管内皮生长因子 (VEGF)与妊高征发病的关系。方法 :收集 1999.2～ 2 0 0 0 .4在我院收治的妊高征 19例作研究对象 ,同期正常孕妇 10例和健康非孕妇 14例作对照。酶联免疫法测定孕妇血清、羊水和新生儿脐血清VEGF浓度。结果 :妊高征和正常孕妇血清VEGF浓度明显高于非孕妇女 (P <0 .0 5 ) ,分别为 6 3.2± 35 .7ng/L ,95 .6± 41.5ng/l和 32 .3± 2 7.0ng/L。妊高征者又明显低于正常孕妇 (P <0 .0 5 ) ,两组脐血VEGF浓度明显高于母血清 (P <0 .0 5 )。两组羊水和脐血VEGF无明显差别。母血、羊水、脐血三者VEGF浓度无明显相关性。结论 :血清VEGF浓度变化可引起内皮损伤、滋养细胞和胎盘血管功能障碍 。

Objective To explore the relationship between the level of vascular endothelial growth factor (VEGF) and pathogensis of Pregnancy Induced Hypertension(PIH).Methods 19 patients with PIH were admitted from February 1999 to April 2000 in our hospital and 10 normal pregnant women and 14 heathy nonpregant women were collected in pairs as controls.Levels of VEGF were measured with enzymonimmunoassay in pergnant women'serum,amniotic fluid and cord serum.Results The levels of serum VEGF were significantly higher in patients with PIH(63.2±35.7 ng/L) and in normal pregant women (95.6±41.5 ng/L) than those in healthy nonpregnant women (32.3±27.0 ng/L)( p <0.05).The levels of serum VEGF in patients with PIH were significantly lower than those in normal pregnant women ( p <0.05),and the levels of serum VEGF in cord serum of two groups were significantly higher than those in maternal serum ( p <0.05).There were no differences in the levels of VEGF between the amniotic fluid and cord blood sampling from two groups.The level of VEGF in maternal blood,amniotic fluid and cord blood had no significant relationship.Conclusion Changes in serum VEGF may result in damage to endothelium and dysfunction of trephocyte and placenta,and then,VEGF plays an important role in the pathogenesis of PIH.