实验性肺纤维化肺灌洗液磷脂组分分析

Phospholipid comoonents in BALFS from Bleomycin induced pulmonary fibrosis in rats

对博莱霉素肺纤维化大鼠模型不同时期作支气管肺泡灌洗液(BALF)细胞汁数与分类、蛋白含量测定和磷脂及其组分测定,并与肺病理变化作对照分析。结果发现BALF细胞计数和分类与肺组织细胞浸润基本一致,BALF蛋白含量增加,磷脂酰甘油(PG)随病变进展而下降,磷脂酰肌醇(PI)和磷脂酰乙醇胺(PE)升高。PG/PI比值下降,PG/PI比值可作为肺纤维化的一种标志。

Qualitative and quantitative analysis was performed on 5 components of phospholipids(PL) isolated from bronchoalveolar lavage fluid (BALF) during the development of Blepmycin induced pulmonary fibrosis in rats with high - performance liquid chromatography (HPLC). The measurement of cells and protein in BALF,the pathologic and morphometric observation with light and electronic microscope were also done. Results indicated that the changes both in BALF cells and in lung histoapthology were similar,the amount of protein in BALF increased, the total amount of PL in BALF increased more than 1. 8 - fold over control animals at days 28 afrer administration of Bleomycin, phosphatidylglycerol (PG) decreased, the PG/PI ratio could be used as a marker for pulmonary fibrosis.