摘要
目的 :研究氧化型胆固醇 (Triol与 2 5 -OH)对人脐静脉内皮细胞凋亡的诱导作用 ,并观察氨氯地平对氧化型胆固醇诱导内皮细胞凋亡的影响。方法 :体外培养人脐静脉内皮细胞 ,利用光镜、电镜、DNA电泳及流式细胞仪测定作为凋亡检测指标。结果 :对照及胆固醇组未检测到细胞凋亡 ,Triol与 2 5 -OH处理组光镜、电镜下可见典型的细胞凋亡形态学改变 ,电泳示“DNAladder” ,流式细胞仪检测出现亚二倍体峰 ,凋亡率分别为 3 2 2 5 %与 2 3 0 4 %,而氨氯地平使其凋亡率分别下降至 13 42 %与 11 77%。结论 :氧化型胆固醇 (Triol与 2 5 -OH)可以诱导人脐静脉内皮细胞发生凋亡 ,而氨氯地平抑制其凋亡诱导作用。
AIM: To investigate the apoptosis of endothelial cells (EC) induced by oxysterols and to examine the effect of amlodipine on the apoptosis induced by oxysterols.METHODS: Light microscope, electron microscope, DNA agarose gel electrophoresis and flow cytometry were used to detect the apoptosis of EC.RESULTS: The characteristic morphological features of apoptosis were observed under light and electron microscope; DNA electrophoresis showed'DNA Ladder'; Flow cytometry showed the sub-G1 peak, apoptotic rate is 32.25% and 23.04% in Triol-treated and 25-OH-treated groups, respectively. While treated EC with amlodipine at the same time, the apoptotic rate decreased significantly. CONCLUSION: Both Triol and 25-OH can induce apoptosis of EC, which can be inhibited by amlodipine.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第2期104-107,共4页
Chinese Journal of Pathophysiology
基金
国家教委博士点科研基金资助项目(No.972 4 )
关键词
胆固醇
内皮
血管
细胞
凋亡
氨氯地平
Cholesterol
Endothelium, vascular
Cells
Apoptosis
Amlodipine
