ICAM-1单抗对烧伤休克大鼠微循环的影响

The effect of intercellular adhesion molecule-1 monoclonal antibody on microcirculation of burn shock in rats

目的 :探讨运用血管内皮细胞粘附分子 (ICAM - 1)单克隆抗体减少白细胞粘附和防治烧伤休克的效应及改善微循环的作用机制。方法 :复制大鼠烧伤休克模型 ,用红细胞跟踪相关仪和电视测微仪测量微静脉血液流速、口径、流量 ,镜下观察细静脉白细胞粘附数 ,并记录动物存活时间。结果 :单抗能减缓烧伤休克大鼠平均动脉压和微静脉血流速度的下降趋势 ,明显减少微静脉中白细胞附壁粘着数 ,使动物的平均存活时间显著延长 ,但仍不足 2 4h。结论 :运用ICAM - 1单抗能阻断白细胞与内皮细胞的粘附 ,减少白细胞嵌塞微静脉 ,达到改善烧伤休克微循环和保护组织细胞的作用 ,但重症休克需要综合治疗。

AIM: To detect the effect of intercellular adhesion molecule-1(ICAM-1) monoclonal antibody on microcirculation disorder in burn shock of Wistar rats. METHODS: The blood flow velocity and diameter of venule were measured with RBC tracking correlator and IV550 model video microscaler in burn shock models of rats. The number of leukocytes adhered on venule wall was calculated under microscope. The animal survival time was observed. RESULTS: ICAM-1monoclonal antibody could attenuate the falling of mean arterial pressure, significantly reduce the number of leukocytes adhered on venule wall, and obviously prolong the animal mean survival time, but less than 24h. CONCLUSION: ICAM-1 monoclonal antibody can decrease the number of adhered leukocytes to endothelial cells, attenuate the tether of leukocytes to venule and improve microcirculation and protect tissue cells in burn shock of rats. However, a comprehensive therapy should be taken in severe shock.