肾上腺素致心肌细胞肥大的机制

ROLE OF ADRENALINE IN THE HYPERTROPHY OF CARDIOMYOCYTES CARDIAC HYPERTROPHY

目的 :探讨肾上腺素致心肌细胞肥大中的机制。方法 :在培养新生大鼠心肌细胞上 ,通过测量心肌细胞表面积和 [3H]-Leu的掺入量来判断心肌细胞肥大。结果 :肾上腺素能明显增加心肌细胞表面积和 [3H]-Leu的掺入量 ,α受体阻滞剂 (酚妥拉明 )、β受体阻滞剂 (心得安 )、百日咳毒素 (PTX)和蛋白激酶C(PKC)抑制剂 (calphostinC)均可抑制肾上腺素诱导的心肌细胞表面积和 [3H]-Leu的掺入量增加。结论 :肾上腺素诱导的心肌细胞肥大与肾上腺能受体 (α受体和 β受体 )激动有关 ,并通过G蛋白和PKC介导。

Objective:The present study was to investigate the role of adrenaline in the hypertrophy of cardiomyocytes.Methods: The measurement of cell surface area and [ 3H]-Leucine incorporation judged the hypertrophy of cardiaomyocyte in cultured neonatal rat myocardal cells.Results:Adrenaline could significantly increase cell surface area and promote [ 3H]-Leucine incorporation. alphal-adrenergic blocking agent(phentolamine), beta-adrenergic blocking agent(propranolol), pertusis toxin(PTX) and PKC inhibitor calphostin C could markedly block effects of adrenaline increasing cell surface area and promoting [ 3H]-Leucine incorporation. Conclusion: These results suggest that the hypertrophy of cardiomyocytes induced by adrenaline in cultured neonatal rat myocardal cells is relation alphal-and beta-adrenergic receptor,PTX sensitive G protien and PKC.