地白忍煎剂对人角朊细胞增殖及抗氧化损伤的作用

The effects of DiBaiRen decoction on the proliferation of human keratinocytes and its in vitro anti-oxidation role

目的 观察地白忍煎剂对人角朊细胞增殖和抗氧化损伤的生物学效应。 方法 体外培养人角朊细胞，分别加入不同浓度的地白忍煎剂，设不同培养时相点计数细胞增殖率并绘制细胞生长曲线，比较细胞增殖速度的变化；在培养液中加入次黄嘌呤-黄嘌呤氧化酶、过氧化氢，模拟超氧阴离子（O2·-）和羟自由基（OH·）对细胞的损伤，应用MTT比色法测定细胞活力，分析地白忍煎剂对自由基损伤的拮抗效应。 结果 加入体积分数为3%～10%的地白忍煎剂后，角朊细胞增殖速度明显加快，细胞融合时间显著缩短；可减轻或消除由次黄嘌呤-黄嘌呤氧化酶、过氧化氢介导的对角朊细胞的氧化损伤反应；地白忍煎剂对角朊细胞产生的生物学效应，在一定浓度范围内呈现剂量依赖性。 结论 地白忍煎剂可促进体外培养的人角朊细胞增殖，具有保护创面促进上皮化并拮抗细胞氧化损伤的作用。

Objective To investigate the effects of DiBaiRen decoction on the proliferation of human keratinocytes and its in vitro anti-oxidation role . Methods Human keratinocytes were cultured in vitro in the media containing gradient diluted DiBaiRen decoction. The cell proliferation rates at different culture times were recorded. Cellular oxidation injury inflicted by superoxide anion and hydroxyl anion was produced by adding hypoxanthine-xanthine oxidase and hydrogen peroxide to the culture media. MTT colorimetry was employed to determine cellular activity.The antagonistic effects of DiBaiRen decoction on cellular oxidation injury were analyzed. Results In the case that DiBaiRen decoction was added to the culture in concentrations of 30ml/L to 100ml/L, there exhibited obvious accelerated proliferation of the keratinocytes,with evident shortening of the cell fusion time. In addition, the oxidation injury of cultured keratinocytes caused by hypoxanthine-xanthine oxidase and hydrogen peroxide was ameliorated or eliminated by the addition of DiBaiRen decoction,and the effects were dose-dependent. Conclusion DiBaiRen decoction might possess anti-oxidation effects and could promote the in vitro proliferation of cultured human keratinocytes,and it might be beneficial in wound protection and epithelization.