紫外线对大鼠胰岛树突状细胞的影响

The effect of ultraviolet on dendritic cell of pancreatic islet of rats

目的 探讨紫外线对大鼠胰岛树突状细胞 (Dendriticcell,DC)结构和功能的影响。 方法 分离大鼠胰岛经紫外线照射后利用免疫组织化学 (ABC)方法显示胰岛Ia+ DC ,透射电镜观察其超微结构 ,同时将处理的胰岛移植于糖尿病小鼠模型。 结果 分离胰岛经紫外线照射后内分泌细胞变化不明显 ,Ia+ DC的突起缩短 ,着色变浅 ,电镜观察胞质中形成抗原肽 MHCⅡ复合物的小泡状结构及吞饮小泡减少 ,异种移植实验胰岛移植存活期 10 .5 4± 2 .0 6天 ,紫外线照射组存活期 17.5 8±1.88天 ,差异有显著性 (P <0 .0 5 )。 结论 适量的紫外线照射对胰岛内分泌细胞无明显损伤 ,DC结构受损 ,功能受到抑制。异种移植实验表明紫外线照射后胰岛移植物存活期延长。

Objective To investigate the effect of ultraviolet on structure and function of dendritic cell (DC) of pancreatic islet of rats. Methods After islets were radiated by ultraviolet Ia positive DC were showed under avidin-biotin-peroxidase complex (ABC) method and ultrastructure was observed by electron microscopy (EM), finally, islet were transplanted into mice with diabetes. Results After pancreatic islet of rats were radiated by ultraviolet, the endocrine cells showed intact morphology and normal structure under light and electron microscopy, but dendrite of Ia positive DC changed from long to short and color of Ia positive DC stained by ABC method was from dark to light. DC showed a decrease in vesiculate structure where antigen peptide-MHC- Ⅱ complex was formed. The survival stage was 10.54±2.06 days in pancreatic islet group, 17.58±1.88 days in ultraviolet radiated group during transplantation experiment. There was a significant difference ( P <0.05). Conclusions A proper amount of ultraviolet might not introduced to damage endocrine cell, but may impair structure and function of DC. The results of survival stage of transplantation has been prolonged for another a few day.