补体活化引起的离体心脏损伤

Isolated guinea-pig working hearts damaged by activation of complements

目的 探讨补体活化是否会直接引起心肌缺血性损伤.方法 20只豚鼠离体工作心脏随机分为A、B二组(每组10只),分别接受3%灭活人血浆+酵母多糖(对照组)、3%人血浆+酵母多糖两种处理,记录二组处理前及处理后15、30、45和60分钟的心外膜心电图以及心输出量(CO)、左心室最大内压(LVP_(max))、左心室内压最大上升速率(dp/dt_(max))和心率(HR),并在实验结束时进行免疫组化检查,观察心肌组织有无C_(5b-9)、C_(3a)沉积.结果 B组在接受处理后心外膜心电图出现ST段抬高,HR增加,CO、LVP_(max)和dp/dt_(max)均下降,这些变化以处理后45分钟最明显:对照组处理前后各项观察指标无明显变化.免疫组化检查:B组可见C_(5b-9)、C_(3a)沉积,而A组无沉积.结论补体活化可直接引起豚鼠工作心脏心肌缺血性损伤.

Objective To determine if activated human complement could directly damaged isolated guinea-pig working hearts. Methods Isolated guinea-pig working hearts perfused with 3% denatured human plasma and zy-mosan in group A (control, n=10) and 3% normal human plasma and zymosan in group B(n= 10), respectively. Of these 2 groups, epicardial electrocardiogram (ECG) and cardiac output (CO), maximum left ventricular develo-peded pressure (LVPmax) ·maximum left ventricular pressure rise rate (dp/dtmax) and heart rate(HR) were recorded before and after 15, 30, 45 and 60 minutes perfusion. After the experiment, immunohistochemical examination was performed to detect the deposition of C3a, and C5b-9 in the myocardium. Results Elevation of ST segment .faster HR and decrease in CO, LVPmax and dp/dtmax were seen in group B, most prominent at 45 minutes after perfusion; no apparent change in all above parameters was seen in group A . Immunohistochemical examination showed the deposition of C3a, and C5b-9 in the myocardium in group B, no deposition of C3a, and C5b-9 in group A. Conclusion Activated human complements directly injured isolated guinea-pig working hearts.