摘要
探讨急性心肌梗死病人单核细胞中IL-6及TNF-α的产生及其临床意义.方法 急性心肌梗死病人组(n=47)、正常对照组(n=13),于急性心肌梗死发病的第1,7,14及21天抽取外周血并分离单核细胞,通过酶联免疫荧光法测定单核细胞培养液中的IL-6及TNF-α浓度.结果 急性心肌梗死组单核细胞培养液中IL-6及TNF-α浓度较对照组明显升高;并在发病第1天前壁心肌梗死较下壁心肌梗死增高有显著意义;单核细胞培养液中IL-6浓度的峰值与血清C-反应蛋白浓度的峰值之间呈显著正相关关系.结论 单核细胞培养液中的IL-6及TNF-α对急性心肌梗死预后的判断有一定意义.
Objective To investigate the production and clinical significance of mononuclear cells (MNCs) related cytokines-interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) in patients with acute myocardial infarction (AMD).Methods 47 patients with AMI and 13 normal controls were studied. Peripheral venous blood MNCs were isolated from patients 1,7,14, and 21 days after onset of AMI. IL-6 and TNF-αlevels in a culture medium of MNCs were measured by enzyme-linked immunosorbent assay. Results IL-6 and TNF-αlevels in a culture medium of MNCs were significantly higher in patients with AMI than in those of control subjects, and the levels were higher in patients with anterior AMI than in patients with inferior AMI. A significant positive correlation was found between peak of IL-6 levels in a culture medium of MNCs and peak of the concentration of C reactive protein in serum . Conclusion IL-6 and TNF-αlevels in a culture medium of MNCs may closely contribute to the prognosis of AMI.
出处
《中国心血管杂志》
2000年第4期198-199,218,共3页
Chinese Journal of Cardiovascular Medicine
