甲硝基亚硝胍诱发大鼠胃癌的实验研究

Experimental Induction of Gastric Carcinoma by N-methyl-N'-nitro-N-nitrosoguandine on Rats

作者自行合成N-甲基-N′-硝基-N-亚硝基胍(MNNG),并以100μg/mlMNNG稀释液喂以100只Wistar大鼠,以诱发胃癌。结果发现喂药6个月内,死亡20只,均无消化道癌肿发生;第7～8个月内,死亡18只,发生胃癌3只(16.7%);第9个月时,处死全部剩余大鼠共62只,其中50只发生胃癌(80.7%)。癌肿均位于腺胃小弯侧,多呈隆起结节型或溃疡型;组织学类型包括乳头状腺癌、分化或未分化管状腺痛;癌肿可侵犯胃壁各层,与人类胃癌病理特征颇为相似。作者还发现,纯系雄性Wistar大鼠是诱发实验性胃癌的理想对象。且以100μg/mlMNNG液,喂药9个月为适宜。

One hundred Wistar rats were given 100 μg/ml solution of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) orally, to establish the animal model gastric carcinoma. After the administration of MNNG solution for 9 months, 50/62 (80.7%) rats developed adenocarcinoma of the stomach. The diagnosis was based on the histoiogic appearances of the lesions. Macroscopically, all the carcinomas were of either polypoid or ulcerated pattern, and developed most frequently at the antrum along the lesser curvature. Microscopically, the gastric wall was involved in various depth. Papillary and tubular adenorcarcinoma were the main histological types while mucous adenorcarcinoma could be found in a few cases. These pathologic types were quite similar to those in the human being. It was concluded that a 9-month administration of 100 μg/ml MNNG solution to the inbred male Wistar rats was a favorite way to establish the animal model of gastric carcinoma.