胃癌细胞DNA流式细胞计分析在胃癌预后中的意义

Prognostic significance of FCM DNA Analysis in Carcinoma of Stomach

对60例手术后随访超过5年以上的进展期胃癌病人的存档石蜡包埋组织按Hedey的方法进行细胞核DNA含量的FCM测定,分析DNA倍体类型与胃癌病人预后的关系。结果为DNA异倍体型胃癌的预后明显较二倍体型胃癌差,与影响胃癌病人预后的其他主要病理形态学因素进行比较发现低分化腺癌、浸润性生长、有淋巴管侵犯及淋巴结转移的胃癌预后分别较高分化腺癌、膨胀型生长、无淋巴管侵犯及无淋巴结转移的胃癌差。胃癌细胞的DNA倍体类型是另一种影响胃癌预后的独立因素。

To explore the correlation between the cellular DNA content, DNA ploidy and prognosis, a flow cytometric (FCM) analysis was performed respectively on paraffin-embedded tissue sections from 60 cases of gastric carcinoma (GC). The results showed that patients with aneuploid tumor in 32/60 (53.3%) of the cases, were associated with significantly poorer prognosis than those with tumor. Univariate analysis also revealed a significant association between patients' survival and the following parameters: degree of differentiation, mode of growth, lymph node metastasis and lymphatic invasion. The results suggested that DNA ploidy was an independent factor of prognostic significance in GC.