细胞因子基因疗法延长烫伤后小鼠同种植皮存活期的实验研究

Effects of cytokine gene therapy on prolonging survival the of allografted skin after scalding in a murine model

目的探讨细胞因子基因疗法对烫伤后同种植皮存活期的影响。方法以小鼠ＩＬ－１０基因为治疗性目的基因，以成纤维细胞为载体细胞，利用基因转录技术，建立成纤维细胞介导的基因治疗延长烫伤小鼠同种植皮存活期的实验模型。利用逆转录病毒载体将ＩＬ－１０转入ＮＩＨ３Ｔ３细胞，小鼠烫伤后创面同种植皮，腹腔内埋植胶原包裹的ＮＩＨ３Ｔ３－ＩＬ－１０细胞，观察其对同种植皮的存活期的影响及小鼠重要脏器的变化。结果细胞因子基因疗法能使同种植皮的存活期明显延长，小鼠重要脏器未见明显病理损害。结论成纤维细胞能成功地将ＩＬ－１０导入体内有效表达，并能抑制机体的免疫应答，延长小鼠烫伤后同种植皮的存活期。

Objective To explore the role of cytokine gene therapy on prolonging survival time of allografted skin after scalding in a muine model. Methods Interleukin 10 (IL-10) gene was employed as therapeutic objective gene and fibroblast was as a carrier cell. Gene transcription technique was adopted to establish an experimental murine model in which fibroblast-mediated gene therapy was used to prolong allografted skin survival time after scalding. IL-10 was transferered into fibroblastocyte (NIH3T3) by reverse transcriptive virus vector. The mice were grafted with allosikin after scalding. In addition, collagen capsulized NIH3T-3-IL-10 cells were implanted intraperitoneally in the mice so as to observe its influence on allografted skin survival time and on the changes of their main internal organs. Results Cytokine gene therapy could obviously prolong the survival time of allografted skin (P < 0.01) without any evident detrimental effect on the internal organs. Conclusion These results demonstrated that skin allograft rejection could be inhibited and the survival time be prolonged with the implantation of the fibroblastocyte transferred IL-10 gene.