金葡菌肠毒素B在烧伤脓毒症大鼠中的分布特点及意义

The sifnificance and characteristics of the internal distribution of Staphylococcal enterotoxin B in burned rats with sepsis

目的 探讨金葡菌肠毒素在烧伤后G+菌脓毒症发生、发展过程中的意义。 方法 采用大鼠 2 0 %TBSAⅢ度烫伤复合金葡菌攻击造成脓毒症模型 ,观察金葡菌肠毒素B(SEB)在动物血浆及心、肝、肺、肾等重要器官中的分布 ,同时动态检测相关器官功能指标的改变。 结果 烫伤脓毒症早期 ,动物血浆中SEB含量呈一过性升高 (P <0 .0 1) ,继而逐渐下降。心、肝、肺、肾等组织中SEB含量于金葡菌攻击后 2h即明显升高 (P <0 .0 1) ,且持续上升。烫伤后 2 4h动物多器官功能明显受损 ,金葡菌的攻击可进一步加重多脏器功能损害 ,且肝、肾等器官损害程度与组织SEB含量呈显著正相关 (P <0 .0 5～ 0 .0 1)。

Objective To investigate the role of staphylococcal enterotoxin B (SEB) in the development of postburn staphylococcus aureus sepsis. Methods Rats inflicted with 20%TBSA Ⅲdegree scald followed by Staphylococcus aureus sepsis were used as the experimental subjects. The distribution of SEB in the vital organs such as the heart, liver, lungs, kidneys and plasma was observed with concomitant serial measurement of the relative organ functional indices. Results The plasma SEB content increased transiently in the early stage of sepsis (P<0.01) and then fell to near that of control group. The SEB contents in the heart, liver, lungs and kidneys increased evidently 2 hours after staphylococcus aureus challenging (P< 0.01) and continued to increase afterwards. The functions of multiple organs were impaired obviously at 24 postburn hours, and the function impairment was aggravated by S. aureus challenge. The degree of organ injury of liver and kidneys correlated positively with tissue content of SEB (P<0.05～0.01). Conclusions Staphylococcal enterotoxin B might play a role in the development of MODS induced by postburn sepsis.