补锌对缺锌大鼠烫伤后营养状态的影响

The effects of zinc supplementation on postburn nutritional status of zinc-deficient scalded rats

目的 观察补锌对缺锌大鼠烫伤后生长及营养状态的影响。 方法 1月龄大鼠进食低锌饲料 1周形成锌缺乏状态 ,造成 2 0 %TBSA深Ⅱ度烫伤后 ,分别改饲低锌、正常锌、高锌饲料。同时设大鼠烫伤前后均进食正常含锌饲料做对照组。用体重变化、累积氮平衡与空肠黏膜、肝、肾、脾、睾丸的DNA和蛋白质含量反映大鼠营养状况。 结果 补正常及高锌大鼠的体重、食物转化率、累积氮平衡和器官蛋白质含量均优于低锌大鼠 ,血清锌、血清碱性磷酸酶活性、空肠黏膜、肾、脾、睾丸的DNA含量在补锌后显著提高。 结论 补锌有助于烫伤后大鼠营养状况的改善 。

Objective To investigate the effects of zinc supplementation on postburn nutritional status of zinc-deficient scalded rats. Methods Weanling Wistar rats were fed with zinc-deficient diets for one week to induce zinc-deficient status. The rats were inflicted with 20% TBSA deep partial thickness burn and thereafter fed with diets of low, normal or high zinc contents. The scalded rats fed with normal zinc-containing diet before and after injury were taken as normal control group. The changes of body weight, cumulative nitrogen balance and the contents of DNA and protein in jejunum mucous, liver, kidneys, spleen and testes were measured to represent the nutritional status of the rats. Results The rats fed with diets with normal and high contents of zinc were superior in status to those with low content of zinc in terms of body weight, food conversion rate, cumulative nitrogen balance and organ protein contents. The serum zinc level, alkaline phosphatase (ALP) activity and the DNA contents in jejunum mucous, kidneys, spleen and testes increased significantly after zinc supplementation . Conclusion Zinc supplementation was helpful in improving postburn nutritional status in scalded rats. And relative higher amount of zinc supplementation might be beneficial to raise the DNA content in testes and kidneys when compared to that with normal zinc supplement.