休克期一次性切痂对巨噬细胞分泌细胞因子的影响

The effects of escharectomy en masse during shock stage on the secretion of cytokines from macrophage in scalded rats.

目的 了解烧伤休克期一次性切痂大鼠血浆对巨噬细胞分泌TNFα、IL - 6和IL - 8的作用及对血浆中炎症介质TNFα、IL - 6和IL - 8影响 ,论证烧伤休克期切痂能否抑制巨噬细胞分泌TNFα、IL - 6和IL - 8。 方法 Ⅲ度 30 %TBSA大鼠随机分为伤后 6h切痂组 (E组 )和烧伤未切痂组 (C组 ) ,检测两组大鼠血浆中TNFα、IL - 6和IL - 8含量 ,并将两组大鼠血浆分别与正常大鼠腹腔巨噬细胞共同孵育 6h ,检测培养液中TNFα、IL - 6和IL - 8含量。 结果 E组和C组伤后血浆中TNFα、IL - 6和IL - 8均明显升高 ,但E组于伤后 12h上述 3项指标下降并明显低于C组。两组大鼠血清均能刺激腹腔巨噬细胞分泌TNFα、IL - 6和IL - 8,但E组的刺激能力明显低于C组。 结论 烧伤休克期一次性切痂 ,能降低烧伤血浆诱导细胞分泌TNFα、IL - 6和IL - 8的能力 ,减少烧伤血浆中TNFα、IL - 6和IL - 8含量 ,有利于防止严重烧伤后炎症反应综合征的发生和发展。

Objective To explore the effects of the plasma from rats undergoing escharectomy en masse during shock stage on the secretion of TNFα,IL-6 and IL-8 by macrophage. Methods Rats inflicted by 30% Ⅲdegree scald were employed as the model. Sixty-three rats were randomly divided into escharectomy (E) and scald (C) groups. The plasma contents of TNFα, IL-6 and IL-8 in rats in two groups were determined. The plasma from rats in two groups was respectively co-cultured with rat peritoneal macrophages for 6 hours and the TNFα, IL-6 and IL-8 in the supernatants of the culture were assayed. Results Plasma contents of TNFα,IL-6 and IL-8 were evidently increased in both E and C groups. But the contents of these cytokines in E group decrease and were much lower than those in C group at 12 postburn hour. The sera from two groups of rats could all stimulate the secretion of TNFα,IL-6 and IL-8 from peritoneal macrophages, but the serum from C group possessed stronger stimulating ability than that from E group. Conclusion Escharectomy en masse during shock stage could weaken the ability of burn sera to induce the secretion of TNFα,IL-6 and IL-8 by macrophages. It was therefore beneficial to the prevention of postburn development of systemic inflammatory response sydrome.