配血用高分子材料的合成与表征

Synthesis and Characterization of Polymer for Blood Matching Test

用烯丙基氯和二甲胺合成了烯丙基氯化铵 ,在引发剂引发下聚合成聚铵阳离子 ,用红外光谱和核磁共振对其结构进行表征。用所合成的高分子材料来检测血清中的不完全抗体IgG ,讨论了EDTA·Na2 浓度、聚铵阳离子浓度、红细胞浓度等对检测红细胞凝聚的影响。结果发现 ,该技术具有快速、简捷和灵敏度高等优点 ,其效果优于传统的方法而和凝聚方法相同。

The quaternary ammonium polyelectrolytes were synthesized by reaction of dimethylamine and allyl chloride, and the structures of monomer and polymer were characterized by infrared and proton nuclear magnetic resonance spectroscopy . The poly(dimethyl diallyl ammonium) chloride was used to detect the incomplete IgG antibodies of red blood cell in human seria, and the several factors, such as concentration of sodium ethylene diamine tetracetic acid(EDTA·Na2), polyelectrolytes and red blood cell were discussed in the match blood test. It is found that this article presents data supporting the use of the manual poly(dimethyl diallyl ammonium )chloride test, a 1-minute room temperature procedures provide a rapid and more sensitive test . It can be compared with bromeline, polybrene technique,etc. The results show that this method is superior to commercial methods, and equal to polybrene . This new technic should be a valuable aid in the detection and identification of weak antibodies.