苹果褪绿叶斑病毒检测:PCR技术与ELISA技术灵敏度比较

Detection of ACLSV by RT-PCR and PAS-ELISA

苹果褪绿叶斑病毒（ＡＣＬＳＶ）是感染仁果类和核果类果树的重要病毒．作者应用ＲＴ－ＰＣＲ技术和ＰＡＳ－ＥＬＩＳＡ技术成功地检测了该病毒，并对指示植物中感染该病毒的检测结果进行了报道．试验结果表明，ＲＴ－ＰＣＲ技术的检测灵敏度是ＰＡＳ－ＥＬＩＳＡ技术的８０００倍．

The optimized RT-PCR system and PAS-ELISA have been used to detect ACLSV. The results show that the relative sensitivity of the optimized RT - PCR system is as 8 000 times as that of PAS-ELISA method when ACLSV is detected.