高粱花粉管通道法导入抗虫基因的研究

The Study on Introducing Insect-resistant Gene into Sorghum Through the Pollen Tube Pathwa

高粱自花受粉后２～６ｈ， 通过花粉管通道将含抗虫基因的重组质粒ＰＡＰＩＤ－ＣｐＴＩ导入到合子内．所得７０２粒种子经１０００ｐｐｍ的卡钠霉素初选后，ＰＣＲ、Ｓｏｕｔｈｅｒｎ检测分别得到１８株、５株阳性个体，阳性率分别为３．１％、０．８６％．结果表明得到了转基因高粱，证明了花粉管通道法可以将抗虫基因导入到高粱体内，获得抗虫的转基因高粱．

The recombinanl plasmids PAP1D-CpTI containing insect-resistant gene are introduced into the zygolyce through the pollen tube pathwa 2-6 hours after the sorghum (sorghum bicolor) are autophilons 702 seeds we get are selected with karnamicin whose concentration is 1000 ppm and the positive rate is 3.10% and 0.86% seperately using PCR detection and southern blotting. The result shows that the transgenic sorghum are produced. It also proves that the transgenic insect-resistant sorghum can be produced by introducing insect-resistant gene into sorghum through the pollen tube pathwa.