A Novel Exponential Kinetic Model for Casein Tryptic Hydrolysis to Prepare Active Peptides

The kinetics of casein tryptic hydrolysis to prepare activepeptides was investigated. Taking into account the reaction mechanismincluding single substrate hydrolysis, irreversible enzymeinactivation, and substrate inhibition, a set of exponentialequations was established to characterize the enzymatic hydrolysiscurves. The verification was carried out by a series of experimentalresults and indicated that the average regressive error was less than5/100. According to the proposed kinetic model, the kinetic constantsand thermodynamic constants of the reaction system were alsocalculated.

The kinetics of casein tryptic hydrolysis to prepare active peptides was investigated. Taking intoaccount the reaction mechanism including single substrate hydrolysis, irreversible enzyme inactivation, and substrateinhibition, a set of exponential equations was established to characterize the enzymatic hydrolysis curves. Theverification was carried out by a series of experimental results and indicated that the average regressive error wasless than 5%. According to the proposed kinetic model, the kinetic constants and thermodynamic constants of thereaction system were also calculated.