营养盐对小球藻生长及胞内蛋白质含量的影响

The effects of nutritions on the C. vulgaris growth and the content of protein

微藻含有的蛋白质、糖蛋白等活性物质具有许多重要的生物活性和生理功能.在24～26℃、自然光照的条件下,首先考察小球藻生长与胞内蛋白质积累的关系,其次利用正交设计实验考察不同浓度的营养盐对小球藻生长及胞内蛋白质含量的影响.结果表明,小球藻中蛋白质的合成与积累主要发生在生长过程的平衡期.利于小球藻生长的营养盐的优化组合为:C6H12O6:2g/L,KNO3:0.8g/L,Na2PO4:0.2g/L,细胞的最大密度为6.46×107个细胞/L培养液.获得最高蛋白质含量的营养盐的优化组合为:C6H12O6:6g/L,KNO3:1.6g/L,NaH2PO4:0.2g/L,与之对应蛋白质含量为:655.4mg/g.此结果为进一步从微藻中获取单一组成的,具有重要生物和生理活性的蛋白质及糖蛋白提供了优化培养微藻的实验依据和规模化生产的基础条件.

Protein and glyooprotein in microalgae have many important biological activities and physiolgical functions. Under the conditions of 24 - 26 ℃ and natural light, the relationship between the growth of C. vulgaris and the accumulation of protein was investigated, and then the effects of nutritions on the C. vulgaris growth and on the contents of protein were studied by means of orthogonal design test. The results have showed that the synthesis and accumulation of protein in C. vulgaris occur in the stationary phase. The Concentration and optimal combination of nutritions depent on the experimental intention. For the C. vulgaris growth, the optimal combination of nutritions is C6H12O6: 2g/L, KNO3: 0.8g/L, NaH2PO4: 0.2g/L, the highest cell density is 6.46×107 cells/L culture; and for the contents of protein, that of nutritions is C6H12O6: 6g/L, KNO3: 1.6g/L, NaH2PO4: 0.2 g/L. The correspond contents of protein is 655.4 mg/g dry weight. The experiment results provide the laboratory foundation and precondition for further optimally culturing C. vulgaris in great scale and further obtaining the important monoforms of protein from C. vulgaris.