摘要
目的 :探讨胚胎兔周围神经低温冷藏后的改变。方法 :以 10 %DMSO(二甲基亚砜 )为低温保护剂 ,采用两步冷冻保存步骤 ,选择 4种不同的冷冻维持温度 (- 2 0℃、- 4 0℃、- 6 0℃、- 80℃ ) ,对孕龄 2 6d～ 2 8d的胎兔坐骨神经进行冷冻 ,维持 30min后 ,放入液氮中保存一周。复温后行四唑盐还原试验、组织学和超微结构观察 ,探讨冷冻维持温度对胎兔周围神经的影响。结果 :- 4 0℃维持温度冷冻后液氮保存的胎兔周围神经代谢活性和超微结构得到良好维持。结论 :两步冷冻法可以较好地保存胎兔周围神经的生物活性 。
Objective:To explore the change in embryonic rabbit's peripheral nerve after cryopreservation.Methods:With 10% dimethlsulfoxide(DMSO) as a cryoprotective agent,embryonic rabbit's sciatic nerve(embryonic age 26d～28d) was stored in liquid nitrogen for one week by a two-step freezing schedule,in which different holding temperature of -20 ℃?-40 ℃?-60 ℃ and -80 ℃ for 30 min were selected in four groups.We probed into the effect of holding temperature on the cryopreserved embryonic nerve according to terazolium test,histological and ultrastructure examination of the thawed nerve.Results:The results show at the holding temperature of -40 ℃ the metablic activity and ultrastructure of the nerves was little changed.Conclusion:The two-step freezing method can preserve the biological activity of the embryonic nerve,which lays a foundation for cryopreservation and transplantation of embryonic nerve.
出处
《解剖与临床》
2002年第3期77-79,共3页
Anatomy and Clinics
