稀土离子及其柠檬酸配合物诱导人红细胞的溶血作用

Study on the hemolysis of human erythrocytes induced by rare earth ion Ln^(3+) and their complex ion [Ln(Cit)_2]^(3-)

目的 :研究稀土离子Ln3 +及其柠檬酸根配合物 [Ln(Cit) 2 ]3 - 诱导人红细胞的溶血作用。方法 :将不同浓度的Ln3 +或 [Ln(Cit) 2 ]3 - 与人红细胞温育后 ,用分光光度法测定离心上清液中的血红蛋白浓度 ,计算相对溶血率H %。用外推法确定临界溶血浓度cH0 。结果 :14种稀土离子对人红细胞的临界溶血浓度cH0 已经确定 ;发现 [Ln(Cit) 2 ]3 - 的H %～c关系曲线为非对称的“钟”型曲线 ,结论 :稀土离子Ln3 +具有较低的临界溶血浓度 ,这说明Ln3 +与红细胞有较强的作用及细胞毒性。 [Ln(Cit) 2 ]3 - 诱导的人红细胞相对溶血率H %与稀土离子在介质中的物种分布有关。

Objective: To study the hemolysis of human erythrocytes induced by rare earth ions Ln 3+ and their complexes [Ln(Cit) 2] 3- (Cit=citric group). Methods: After incubation of Ln 3+ or [Ln(Cit) 2] 3- ions with human erythrocytes, hemoglobin in supernatant was determined by spectrophotometry, relative hemolytic rates H% of erythrocytes at various concentrations of Ln 3+ or [Ln(Cit) 2] 3- ions were calculated, and the critical hemolytic concentration c H 0 was estimated with extrapolation of H%- [Ln(Cit) 2] 3- curves.Results :The c H 0 values of 14 kinds of rare earth ions were determined and found for the first time the asymmetrical 'bell' type curve in the relationship H% of [Ln(Cit) 2] 3- to their concentrations ( c ). Conclusion: The fact that rare earth ions Ln 3+ have low critical hemolytic concentration indicates Ln 3+ acts strongly to erythrocytes and exhibit obvious cellular toxicity. The relative hemolytic rare H% of [Ln(Cit) 2] 3- depends on the species distribution of rare earth ions in the incubation medium.