肠道B细胞淋巴瘤Epstein-Barr病毒感染与癌基因

Investigation of the Epstein-Barr virus infection and oncogene in intestinal B-cell lymphoma

目的 :研究肠道非霍奇金淋巴瘤B细胞型的Epstein Barr(EB)病毒感染、p5 3、p2 1ras基因表达及其相关性。方法 :应用单克隆抗体、免疫组化方法对瘤细胞进行免疫表型研究 ,CD2 0、CD45RA、CD79a鉴定瘤细胞的B细胞分化 ,CD45RO、CD3除外瘤细胞T细胞分化。同时用免疫组化方法 ,检测瘤细胞 p5 3基因表达、p2 1ras基因表达。EB病毒寡核苷酸探针 (EBER)原位杂交 ,观察瘤细胞EB病毒感染情况。结果 :34例肠道B细胞淋巴瘤好发部位为回肠和结肠 ,以单发瘤结节多见 ,常伴有表面溃疡形成。 34例中 ,弥漫性大B细胞淋巴瘤 9例 (2 6 .5 % ) ,19例为惰性的边缘区B细胞淋巴瘤黏膜相关淋巴组织型 (MZL MALT型 ) (5 5 .9% ) ,6例为MZL MALT型伴高度恶性转化(17.6 % )。EBV EBER原位杂交检测 ,全部病例为阴性。p5 3蛋白表达共有 2 3例 ,占全部病例的 6 7.6 % (其中高度恶性占 47.8% ) ;16例有 p2 1ras的表达 ,为 47.1% (其中高度恶性占 43.8% )。有 15例同时检出 p5 3蛋白和p2 1ras的表达 ,占病例数的 44 .1%。结论 :34例肠道B细胞淋巴瘤以惰性为多见 ,但恶性转化明显。EBV的研究提示肠道B细胞淋巴瘤有别于T细胞淋巴瘤 ,与EBV感染无关。p5 3蛋白的表达高于 p2 1ras,p5 3和 p2 1ras的共同表达检出率较高 ,提示癌基因 p5

Objective: To study the relationship among Epstein Barr virus (EBV) infection, p53 protein and p21 ras expression in intestinal B cell lymphoma. Methods :The immunophenotype of tumor cells, CD20?CD45RA?CD79a for B cell,CD45RO?CD3 for T cell, p53(DO 7) and p21 ras for oncogene were studied using some antibodies and immunohistochemistry methods, in situ hybridyzation was used for detection of EBV encoding small nuclear RNA(EBER). Results:34 cases of intestinal B cell lymphomas often located at lower part of lieum and colon, and most of them were single mass with ulcer on its surface. Of 34 cases of intestinal B cell lymphomas ,9 cases(26.5%) were diffuse large B cell lymphomas, 19 cases(55.9%) were marginal zone B cell lymphoma, mucosa associated lymphoid tissue type(MZL MALT), which were belong to indolent, and 6 cases transferred to high malignancy from indolent (17.6%). All of the cases were negative for EBV EBER in situ hybridization. p53 protein were overexpressed in 23 cases (67.6%), near the half cases were high malignancy. 16(47.1%) cases were positive with p21 ras , about 43.8% of which were high malignancy. 15 cases were expressed both with p53 protein and p21 ras . Conclusion :34 cases of intestinal B cell lymphoma were more often MZL MALT type, but with high malignancy. EBV results showed that intestinal B cell lymphomas were different from intestinal T cell lymphomas, and they had no relationship with EBV infection. The expression of p53 protein was higher than that of p21 ras . The detective rate of co expression of p53 protein and p21 ras was high, indicating that p53 protein and p21 ras play an important role in the formation of intestinal B cell lymphoma.