荧光定量聚合酶链反应在检测乙肝孕妇患者血清HBV-DNA及母婴传播中的临床应用

Application of Fluorescence Quantitative PCR in Detection of Hepatitis B Virus in the Pregnant Women With Type B Hepatitis and Study of Intrauterine Infection

目的 探讨乙型肝炎感染孕妇血清HBV -DNA含量和乙肝标志物与胎儿宫内感染的关系 ,同时针对高危人群采取适当措施 ,以降低宫内感染的发生率。方法 用实时荧光定量聚合酶链反应 (FQ -PCR)方法 ,检测了 5 9例乙肝感染孕妇血清以及其胎儿股静脉血的HBV -DNA的含量。结果 乙肝孕妇血清HBeAg阳性组HBV -DNA含量明显高于抗 -Hbe和抗HBc阳性组 ,而后两组中部分病例HBV -DNA水平仍很高。胎儿发生宫内感染与其母亲血清HBV -DNA含量有关 ,随着孕妇血清中HBV -DNA含量的增高 ,胎儿发生宫内感染的危险性也呈增高的趋势。结论 定量PCR检测血清HBV -DNA水平对了解乙肝孕妇患者病毒复制和孕妇传染性的强弱与母婴传播的关系具有一定指导作用 ,采取适当措施 。

Objective To study the relationship of hepatitis B virus quantitative,serum HBV marker in type B hepatitis pregnant women and the intrauterine infection of their newborns.Methods Serum DNA were quantitatively determined by fluorescence quantitative PCR(FQ-PCR) in 59 pregnant women infected with hepatitis B virus and their newborns. Results Serum HBV-DNA concentration in type B hepatitis pregnant women with HBeAg positive was significantly higher than that in those with anti-HBe and anti-HBc positive,but the tatter two groups,the serum HBV-DAN level was still higher in some cases,rate of intrauterine fetal infection was associated with serum HBV-DNA content of gravida,the dangerous of intrauterine infection was increased with maternal HBV-DNA level elevation. Conclusions The HBV-DNA quantitative detection has an instructive role in understanding the relationship between the hepatitis virus duplication level in pregnant women and the intrauterine infection rate.With proper measure,we can reduce the occurrence rate of intrauterine infection.