仙杞煎液对实验性骨质疏松大鼠骨密度、白介素6和血浆酸性磷酸酶的影响

Effect of Xianqi Decoction on bone mass density. IL-6 and plasma acid phosphatase in experimental osteoporosis rats

目的:探讨仙杞煎液对大鼠实验性骨质疏松症的治疗作用及其作用机理。方法:选用7月龄SD雌性大鼠72只,随机分为2组。手术组60只行双侧卵巢切除术,假手术组12只行伪手术,复制成绝经后骨质疏松大鼠模型。将手术组随机分为模型组、西药组、仙杞煎液低、中、高剂量组,连同假手术组共6组按相应剂量灌胃给药。60天后摘眼球取血后将大鼠处死。分离血清和血浆,测定血清白细胞介素6(IL-6)和血浆酸性磷酸酶(ACP)水平,测大鼠脊柱和股骨骨密度。结果:与模型组相比,各中药组可显著降低IL-6和PCP水平(P<0.05或P<0.01).中药各剂量组及西药组间差异无显著性(P>0.05)。与模型组相比,各用药组骨密度均升高(P<0.05)。

Objective: To investigate the effect of Xianqi Decoction on experimental osteoporosis of rats and its mechanism. Methods: 72 female SD rats aged 7 months were randomly divided into two groups, i. e. 60 for operation group, 12 for sham-operation group. Bilateral ovariectomy was performed on operation group. 2 months later, postmenopausal osteoporosis model rats were successfully established. Rats in operation group were randomly divided into model group, western medicine group, Xianqi Decoction low dose group, middle dose group and high dose group. Total six groups were given corresponding medicine by intragastric perfusion. 60 days later, rats were sacrificed after taking blood sample. Serum IL-6. plasma acid phosphatase (ACP) and bone mass density of vertebra and femur were determined. Results: Compared with model group. IL-6 and ACP level in three Xianqi Decoction groups was significantly reduced (P<0. 05 or P<0. 01). and there was no significant difference among Xianqi Decoction groups and western medicine group. Bone mass density in all drug-administered groups was increased (P<0. 05).