苯酚降解菌的筛选及其降解特性的初步研究

SCREENING AND CHARACTERIZATION OF A PHENOL-DEGRADING BACTERIUM

从某印染厂下水道的污泥中分离到一株能高效降解苯酚的菌株ph1 6,经初步鉴定为微球菌属 (Micrococcussp )。该菌株最高可耐受 1 5g L左右的苯酚 ,对苯酚降解最适条件为pH 7 0 ,温度 35℃ ,苯酚浓度为 1 0g L ,时间为 36h,降解率可达 99 6 %。试验还表明Hg+ 、Co2 + 、Ag2 + 等重金属离子对该菌株降解苯酚能力有不同程度的抑制作用。并对其降解动力学作了初步探讨。

A strain ph 16 , that could effectively degrade phenol,was isolated from sewer sludge of printing and dyeing plant. The preliminary identification sugg ested that the strain belongs to Micrococcus sp. The strain could resist to phenol up to 1.5 g/L. The efficient biodegradation of phenol occurred when th e strain was cultured in the medium (pH 7.0) containing 1.0 g/L phenol under 35℃, wh er e the highest degradation rate reach 99.6% after 36 hours. This strain, when t re ated with some heavy metal ions such as Hg +、Co 2+ and Ag 2+ , showe d the significant inhibition of phenol degradation by 74.2%～100%. The kinetic s of phenol degradation during culture of the strain was also explored.