摘要
目的 探讨腺苷与单磷酯A联合预处理对供心保存的效果。方法 健康大白兔 39只 ,随机分为 4组。A、B组以单磷酯A预处理 2 4h后 ,制成离体心脏 ,A组再用腺苷预处理 ;C组用腺苷作经典预处理 ;D组仅注射生理盐水作对照。各组预处理后 ,用 4℃改良St.Thomas液诱导心脏停搏 ,低温保存 4h ,复灌 1h。观察心功能恢复率、心肌三磷酸腺苷 (ATP)和丙二醛 (MDA)含量、心肌磷酸肌酸激酶 (CK mb)释放量等。结果 A、B、C、D组心功能恢复率 (+dp/dtmax ,% )分别为 :70 .97± 17.92 ,6 5 .5 4± 2 2 .6 2 ,6 4 .36± 16 .10 ,39.0 7± 13.78;A组高于B、C、D组 ,并与D组的差异有显著性 (P <0 .0 1)。A、B、C、D组心肌组织ATP含量 (10 -3 μmol/g湿重 )分别为 :5 .4 6± 1.37,3.97± 1.0 4 ,4 .4 5± 1.2 9,2 .0 7± 0 .74 ;A组高于B、C、D组 ,差异有显著性 (P <0 .0 5orP <0 .0 1)。结论 用单磷酯A和腺苷联合预处理 ,对供心有一定的保护效果。
Objective To study the feasibility of additive improvement of heart protection by preconditioning with MLA and adenosine.Methods Rabbits of group A were injected with MLA, then 24?h later the hearts were isolated, mounted on a Langendorff apparatus, preconditioned with adenosine, perfused with filtered modified Krebs-Henseleit(K-H) buffer through left atria, instrumented left ventricular function monitor system and paced ventricularly at 180 beat/min. Hearts were arrested with 4?℃ modified St.Thomas solution, stored for 4?h at 4?℃ in this solution, and reperfused with K-H buffer. Left ventricular function, myocardial CK-mb leakage and tissue levels of adenosine triphosphate were measured. Groups B and C were only preconditioned with MLA or adenosine, respectively, while group D was only preserved with the modified St.Thomas solution in 4?℃.Results The left ventricular function recovery as percentage (+dp/dt max) in groups A, B, C was 70.97 ± 17.92 , 65.54 ± 22.62 , 64.36 ± 16.10 , respectively. If compared with group D ( 39.07 ± 13.78 ), the differences were significant ( P < 0.01 ). Myocardial ATP content in group A was much higher than groups B, D and C ( P < 0.01 or P < 0.05 ). Conclusion This study suggested myocardial delayed preconditioning with MLA might be partially enhanced by the early preconditioning with adenosine and have more beneficial effects on donor heart preservation.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2003年第6期338-340,共3页
Chinese Journal of Organ Transplantation
